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Image Search Results
Journal: bioRxiv
Article Title: Analysis of the hypothalamic oxytocin system and oxytocin receptor-expressing astrocytes in a mouse model of Prader-Willi syndrome
doi: 10.1101/2022.08.15.503958
Figure Lengend Snippet: (A) Schematic depiction of the transgenic modification in Magel2 tm1 . Stw mice; insertion of the LacZ B-Gal gene results in expression of a fused Magel2 protein (B) Images show presence of LacZ reporter (X-gal staining) in Magel2 tm1 . Stw mice. (C) Schematic depiction of coronal sectioning and anatomical location of the PVN. (D) Side-to-side comparison of OT neurons in the PVN of WT and Magel2 tm1 . Stw mice at various Bregma levels. Scale bar=100µm. (E) No difference in OT signal intensity between WT and Magel2 tm1 . Stw mice (n=5 per group). (F) Imaris-assisted, automated cell counting of PVN OT neurons via the spot function. raw image of PVN OT neurons, each spot represents one OT neuron, co-localization of OT neurons and spots, high magnification image showing placement of spots in the OTergic soma. (G) Quantification of OT cell numbers within the PVN in male and female mice. Significant differences were observed in the total number of OT cells and the caudal PVN (parvocellular subdivision, n=5 per group). (H) Depiction of the pipeline for three-dimensional reconstruction of OT neurons based on fluorescent immunolabeling. High magnification insets highlight the detailed reconstruction of OTergic somata. (I) No difference in OTergic morphology between WT and Magel2 tm1 . Stw mice (n=5 per group). *p<0.05, **p<0.01, one-way ANOVA.
Article Snippet: Stw mice using our previously established,
Techniques: Transgenic Assay, Modification, Expressing, Staining, Comparison, Cell Counting, Immunolabeling